Circumcision Info!...
- Thread starter yulia
- Start date
COSMETICS:musicmom said:
According to this article: http://www.oprah.com/health/beauty/h...eatments.jhtml[/URL]
TNS Recovery Complex is engineered from human foreskins--"Dr. Wexler also mentioned a new product that boosts collagen production and can rejuvenate skin called TNS Recovery Complex. TNS is comprised from six natural human growth factors found in normal healthy skin. Dr. Wexler told us the factors are engineered from human foreskin!"
According to this site, which is the main site of the TNS complex:
http://www.skinmedicaaesthetics.com/...covery-complex[/URL]
The priniciple ingredient is Nouricel-MD
And according to this site: http://www.hirshdermatology.com/nouricelmd.htm[/URL]
" Nouricel-MD is a unique product that consists of a concentration of naturally occuring human growth factors derived from bio-engineered tissue cultures. These growth factors help to promote collagen production, new blood vessel growth,thickening of the epidermis, and to decrease inflammation. "
http://or-live.mediwire.com/main/Def...rticleID=24844[/URL]
"The product (TNS Recovery Complex with NouriCel-MD) contains a concentrated formulation of the nutrient solution
derived from manufacture of bioengineered human tissue using neonatal fibroblasts. "
"TNS Recovery Complex with NouriCel-MD is a product of SkinMedica, Carlsbad, Calif. The enriched tissue nutrient solution used in its manufacture is obtained from Advanced Tissue Sciences. Dr. Fitzpatrick is founder and chairman of the board of SkinMedica."
Looks like SkinMedica took over ATS in 2003.
More links:
http://www.naturalfamilyawareness.com/blog/?p=273[/URL]
Cosmetics companies, such as Estee Lauder, Helene Curtis, and Mary Kay Cosmetics, use babies' foreskins in their test laboratories.
There are at least 3 types of cell cultures derived from foreskin. Keratinocytes, Melanocytes, and Fibroblasts.
There are over 30,000 hits for "melanocyte, foreskin" on google.
http://www.google.co.uk/search?biw=9...=Google+Search[/URL]
-----------------------------------------------------------------------------------------------------------------------------
"Apligraf is manufactured under aseptic conditions from human neonatal male foreskin tissue."
http://www.pharma.us.novartis.com/pr...f/apligraf.pdf[/URL]
http://www.organogenesis.com/product.../testskin.html[/URL] - used for testing cosmetics, interestingly they have taken out the reference on the site to what this product is made of, perhaps they didn't like the negative publicity. However, they have to tell the people who use it what it is made of, and one of those documents is here:
"Application
artificial skin for toxicological
tests
®
(Testskin II by
Organogenesis, USA)
Cell Type
neonatal fibroblasts and
keratinocytes (human)
References
www.organogenesis.com[/URL]
Application
collagen-based skin
equivalents for cutaneous
irritation, phototoxicity and
photoprotection studies
Cell Type
neonatal fibroblasts and
keratinocytes (human)
References
Damour 1998 "
From the table on page 28 in the Introduction.
http://www.pharmtech.uni-erlangen.de/Diss_Geiger.pdf[/URL]
Organogenesis Inc Annual Report:
http://www.secinfo.com/dS997.41yp.htm[/URL]
http://sec.edgar-online.com/2001/04/...2/Section2.asp[/URL]
"WE MUST BE ABLE TO OBTAIN ADEQUATE SOURCES OF SUPPLY
We manufacture Apligraf for commercial sale, as well as for use in clinical trials, at our Canton, Massachusetts facility. Among the fundamental raw materials needed to manufacture Apligraf are keratinocyte and fibroblast cells. Because these cells are derived from donated infant foreskin, they may contain human-borne pathogens. We perform extensive testing of the cells for pathogens, including the HIV or "AIDS" virus. Our inability to obtain cells of adequate purity, or cells that are pathogen-free, would limit our ability to manufacture sufficient quantities of our products. "
"....Interruptions in our supply of materials may occur in the future or we may have to obtain alternative vendors for these materials. Any significant supply interruption would adversely affect the production of Apligraf. In addition, an uncorrected impurity or a supplier's variation in a raw material, either unknown to us or incompatible with our manufacturing process, could hurt our ability to manufacture products. "
They also clearly don't realise that everything on the net is archived, lol. The 2002 product page for TestSkin II is here:
http://web.archive.org/web/200209292...nogenesis.com/[/URL]
This brings up the front page, click on "Products and Programs" on left hand menu bar , then "TestSkin II", then "Technical Information" - tiny writing menu just above giant TestSkin II logo in centre of page, then "Physical Characteristics of TESTSKIN II Units."
---------------------------------------------------------------------------------------------------------------------------
http://www.cellapplications.com/HumanCells/HEM.htm[/URL]
Another site's info on Melanocytes. Quote:
"Human Epidermal Melanocytes (HEM) are primary cells isolated from normal human neonatal foreskin."
http://www.freepatentsonline.com/5589161.html[/URL]
Page on Melanocyte preparation.
http://www.cascadebio.com/[/URL]
More melanocyte foreskins for sale here.
ABOUT FIBROBLASTS.
A Google search produces 21,300 hits for fibroblast foreskin.
http://www.ncbi.nlm.nih.gov/entrez/q...&dopt=Abstract[/URL]
"Fibroblast cultures derived from neonatal foreskin explants have been an important model for understanding the basic mechanisms of fibroblast function and activity. Neonatal foreskin has been routinely usedfor establishing such fibroblast cultures in vitro."
"We found that outgrowth and propagation of viable fibroblasts in vitro occurred with foreskin samples obtained within 24 days after surgical harvesting."
HARVESTING???
Watch for the words keratinocyte, melanocyte, fibroblast. If mentioned, ask from what the cells are derived.
To find the products on the Cascade Biologics site, you can do a search for "foreskin" and the products will come up.
http://www.cascadebio.com/[/URL]
Cells for sale:
http://www.cascadebio.com/US/product...id=100&pid=270[/URL]
(that's Keratinocytes)
http://www.cascadebio.com/US/product...id=101&pid=193[/URL]
(Melanocytes.)
http://www.cascadebio.com/US/product...id=149&pid=236[/URL]
(neonatal endothelial cells from neonatal dermis)
http://www.cascadebio.com/US/product...id=102&pid=204[/URL]
(Fibroblasts)
There are more types of cells for sale, but I'm sure you get the picture.
http://www.cambrex.com/Content/biosc...id=NHEM&noCK=Y[/URL]
At this website you can BUY foreskin-derived melanocytes.
2007/06/16 - Cambrex have sold the Research Products and Microbial Biopharmaceutical business to Lonza.
http://www.lonzabioscience.com/conte...elltherapy.asp[/URL]
Neonatal Fibroblasts:
http://www.lonzabioscience.com/Lonza...rodoid.NHDFneo[/URL]
Neonatal Keratinocytes:
http://www.lonzabioscience.com/Lonza...odoid.NHEK-neo[/URL]
Neonatal Melanocytes:
http://www.lonzabioscience.com/Lonza...0.prodoid.NHEM[/URL]
The melanocyte poster is now here:
http://www.lonzabioscience.com/Conte...ss.207.id.1680[/URL]
" Materials and Methods
Cell Sources
NHEM are isolated neonatal foreskin tissue resulting from routine circumcision. All samples are tested via PCR for HIV DNA and Hepatitis B and C DNA. These tissues are processed using mechanical and enzymatic methods. Once the cells are liberated from the tissue, they are cultured in a proprietary Melanocyte Isolation Medium through two passages "
Cell lines do not last forever and need to be replaced constantly - from the instruction sheet from Lonzo (previously Cambrex, and obviously still using their documentation)
Instruction sheet:
CULTURES HAVE A FINITE LIFESPAN IN VITRO.
Cambrex warrants its cells in the following manner
only if Clonetics media and reagents are used.
1. Clonetics NHEK cryopreserved cultures are
assured for experimental use for fifteen
population doublings.
2. Clonetics NHEK proliferating cultures are
assured for experimental use for ten population
doublings.
3. Additional population doublings and subcultures
are possible, but growth rate, biological
responsiveness and function deteriorate with
subsequent passage.
4. NHEK can become irreversibly contact-inhibited
if allowed to reach confluence. To avoid the loss
of your cells and forfeiture of your warranty,
subculture cells before they reach 80%
confluence.
http://www.lonzabioscience.com/Conte...ass.193.id.745[/URL]
(pdf)
There are over 30,000 hits for "melanocyte, foreskin" on google.
http://www.google.co.uk/search?biw=9...=Google+Search[/URL]
-----------------------------------------------------------------------------------------------------------------------------
"Apligraf is manufactured under aseptic conditions from human neonatal male foreskin tissue."
http://www.pharma.us.novartis.com/pr...f/apligraf.pdf[/URL]
http://www.organogenesis.com/product.../testskin.html[/URL] - used for testing cosmetics, interestingly they have taken out the reference on the site to what this product is made of, perhaps they didn't like the negative publicity. However, they have to tell the people who use it what it is made of, and one of those documents is here:
"Application
artificial skin for toxicological
tests
®
(Testskin II by
Organogenesis, USA)
Cell Type
neonatal fibroblasts and
keratinocytes (human)
References
www.organogenesis.com[/URL]
Application
collagen-based skin
equivalents for cutaneous
irritation, phototoxicity and
photoprotection studies
Cell Type
neonatal fibroblasts and
keratinocytes (human)
References
Damour 1998 "
From the table on page 28 in the Introduction.
http://www.pharmtech.uni-erlangen.de/Diss_Geiger.pdf[/URL]
Organogenesis Inc Annual Report:
http://www.secinfo.com/dS997.41yp.htm[/URL]
http://sec.edgar-online.com/2001/04/...2/Section2.asp[/URL]
"WE MUST BE ABLE TO OBTAIN ADEQUATE SOURCES OF SUPPLY
We manufacture Apligraf for commercial sale, as well as for use in clinical trials, at our Canton, Massachusetts facility. Among the fundamental raw materials needed to manufacture Apligraf are keratinocyte and fibroblast cells. Because these cells are derived from donated infant foreskin, they may contain human-borne pathogens. We perform extensive testing of the cells for pathogens, including the HIV or "AIDS" virus. Our inability to obtain cells of adequate purity, or cells that are pathogen-free, would limit our ability to manufacture sufficient quantities of our products. "
"....Interruptions in our supply of materials may occur in the future or we may have to obtain alternative vendors for these materials. Any significant supply interruption would adversely affect the production of Apligraf. In addition, an uncorrected impurity or a supplier's variation in a raw material, either unknown to us or incompatible with our manufacturing process, could hurt our ability to manufacture products. "
They also clearly don't realise that everything on the net is archived, lol. The 2002 product page for TestSkin II is here:
http://web.archive.org/web/200209292...nogenesis.com/[/URL]
This brings up the front page, click on "Products and Programs" on left hand menu bar , then "TestSkin II", then "Technical Information" - tiny writing menu just above giant TestSkin II logo in centre of page, then "Physical Characteristics of TESTSKIN II Units."
---------------------------------------------------------------------------------------------------------------------------
http://www.cellapplications.com/HumanCells/HEM.htm[/URL]
Another site's info on Melanocytes. Quote:
"Human Epidermal Melanocytes (HEM) are primary cells isolated from normal human neonatal foreskin."
http://www.freepatentsonline.com/5589161.html[/URL]
Page on Melanocyte preparation.
http://www.cascadebio.com/[/URL]
More melanocyte foreskins for sale here.
ABOUT FIBROBLASTS.
A Google search produces 21,300 hits for fibroblast foreskin.
http://www.ncbi.nlm.nih.gov/entrez/q...&dopt=Abstract[/URL]
"Fibroblast cultures derived from neonatal foreskin explants have been an important model for understanding the basic mechanisms of fibroblast function and activity. Neonatal foreskin has been routinely usedfor establishing such fibroblast cultures in vitro."
"We found that outgrowth and propagation of viable fibroblasts in vitro occurred with foreskin samples obtained within 24 days after surgical harvesting."
HARVESTING???
Watch for the words keratinocyte, melanocyte, fibroblast. If mentioned, ask from what the cells are derived.
To find the products on the Cascade Biologics site, you can do a search for "foreskin" and the products will come up.
http://www.cascadebio.com/[/URL]
Cells for sale:
http://www.cascadebio.com/US/product...id=100&pid=270[/URL]
(that's Keratinocytes)
http://www.cascadebio.com/US/product...id=101&pid=193[/URL]
(Melanocytes.)
http://www.cascadebio.com/US/product...id=149&pid=236[/URL]
(neonatal endothelial cells from neonatal dermis)
http://www.cascadebio.com/US/product...id=102&pid=204[/URL]
(Fibroblasts)
There are more types of cells for sale, but I'm sure you get the picture.
http://www.cambrex.com/Content/biosc...id=NHEM&noCK=Y[/URL]
At this website you can BUY foreskin-derived melanocytes.
2007/06/16 - Cambrex have sold the Research Products and Microbial Biopharmaceutical business to Lonza.
http://www.lonzabioscience.com/conte...elltherapy.asp[/URL]
Neonatal Fibroblasts:
http://www.lonzabioscience.com/Lonza...rodoid.NHDFneo[/URL]
Neonatal Keratinocytes:
http://www.lonzabioscience.com/Lonza...odoid.NHEK-neo[/URL]
Neonatal Melanocytes:
http://www.lonzabioscience.com/Lonza...0.prodoid.NHEM[/URL]
The melanocyte poster is now here:
http://www.lonzabioscience.com/Conte...ss.207.id.1680[/URL]
" Materials and Methods
Cell Sources
NHEM are isolated neonatal foreskin tissue resulting from routine circumcision. All samples are tested via PCR for HIV DNA and Hepatitis B and C DNA. These tissues are processed using mechanical and enzymatic methods. Once the cells are liberated from the tissue, they are cultured in a proprietary Melanocyte Isolation Medium through two passages "
Cell lines do not last forever and need to be replaced constantly - from the instruction sheet from Lonzo (previously Cambrex, and obviously still using their documentation)
Instruction sheet:
CULTURES HAVE A FINITE LIFESPAN IN VITRO.
Cambrex warrants its cells in the following manner
only if Clonetics media and reagents are used.
1. Clonetics NHEK cryopreserved cultures are
assured for experimental use for fifteen
population doublings.
2. Clonetics NHEK proliferating cultures are
assured for experimental use for ten population
doublings.
3. Additional population doublings and subcultures
are possible, but growth rate, biological
responsiveness and function deteriorate with
subsequent passage.
4. NHEK can become irreversibly contact-inhibited
if allowed to reach confluence. To avoid the loss
of your cells and forfeiture of your warranty,
subculture cells before they reach 80%
confluence.
http://www.lonzabioscience.com/Conte...ass.193.id.745[/URL]
(pdf)
Other sites of interest (gleaned from John Hopkins Center Altweb http://altweb.jhsph.edu/[/URL] it's a huge site, and I don't have time to search it all, but there is a huge amount of work going on using neonatal foreskin in in order to save animals from being used in testing):
http://www.invitroderm.com/[/URL]
"Alternatives to Skin Irritation/Corrosion Testing in Animals"
Go to "search abstracts" http://webglimpse.net/cgi-bin/webglimpse.cgi?ID=36[/URL]
and put in "neonatal foreskin"
5 results, all research papers using cells derived from neonatal foreskins.
http://invitroderm.com/abstracts/slivka2.html[/URL]
http://invitroderm.com/abstracts/cannon.html[/URL]
http://invitroderm.com/abstracts/cannon2.html[/URL]
<I>"A model of the human epidermis, EpiDermÔ , cultured from neonatal foreskin-derived normal human epidermal keratinocytes (NHEK) was introduced by MatTek Corporation in April 1993. Using highly controlled tissue culture techniques, weekly batches of EpiDerm are produced and shipped domestically and internationally for dermal irritancy testing, percutaneous absorption studies, and basic skin research, amongst other uses."</I>
http://invitroderm.com/abstracts/slivka.html[/URL]
http://invitroderm.com/abstracts/lake.html[/URL]
<I>"The </I>[/COLOR]<I> Department of Transportation now requires companies to assess the skin corrosivity of raw materials and products in order to insure their safe transport. The United Nations has issued guidelines and assigned packaging groups to materials based on how quickly corrosion of skin occurs in animal studies: I: less than 3 minutes, II: 3 minutes to 1 hour, III: 1 hour to 4 hours, and Non-corrosive: greater than 4 hours. It was anticipated that EpiDermÔ , a model of the epidermis cultured from neonatal foreskin-derived normal human epidermal keratinocytes would be useful in predicting skin corrosivity since the barrier properties of EpiDerm have been shown to closely approximate those of normal skin."[/COLOR]</I>
http://www.invitroderm.com/[/URL]
"Alternatives to Skin Irritation/Corrosion Testing in Animals"
Go to "search abstracts" http://webglimpse.net/cgi-bin/webglimpse.cgi?ID=36[/URL]
and put in "neonatal foreskin"
5 results, all research papers using cells derived from neonatal foreskins.
http://invitroderm.com/abstracts/slivka2.html[/URL]
http://invitroderm.com/abstracts/cannon.html[/URL]
http://invitroderm.com/abstracts/cannon2.html[/URL]
<I>"A model of the human epidermis, EpiDermÔ , cultured from neonatal foreskin-derived normal human epidermal keratinocytes (NHEK) was introduced by MatTek Corporation in April 1993. Using highly controlled tissue culture techniques, weekly batches of EpiDerm are produced and shipped domestically and internationally for dermal irritancy testing, percutaneous absorption studies, and basic skin research, amongst other uses."</I>
http://invitroderm.com/abstracts/slivka.html[/URL]
http://invitroderm.com/abstracts/lake.html[/URL]
<I>"The </I>[/COLOR]<I> Department of Transportation now requires companies to assess the skin corrosivity of raw materials and products in order to insure their safe transport. The United Nations has issued guidelines and assigned packaging groups to materials based on how quickly corrosion of skin occurs in animal studies: I: less than 3 minutes, II: 3 minutes to 1 hour, III: 1 hour to 4 hours, and Non-corrosive: greater than 4 hours. It was anticipated that EpiDermÔ , a model of the epidermis cultured from neonatal foreskin-derived normal human epidermal keratinocytes would be useful in predicting skin corrosivity since the barrier properties of EpiDerm have been shown to closely approximate those of normal skin."[/COLOR]</I>
AltBIB - Bibliography on Alternatives To Animal Testing:
http://toxnet.nlm.nih.gov/altbib.html[/URL]
In the search field I put in "neonatal Foreskin" 5 results:
Unfortunately, the search results are only held in a temp file, so they don't work when the search expires. I've given the information on how to get there, and I hope it will be possible for other people to easily pull the abstracts up.
1. <I>"CHARACTERIZATION OF AN IMMORTALIZED HUMAN CELL LINE DERIVED FROM NEONATAL FORESKIN DIPLOID FIBROBLASTS
Authors: Chiang LC Chiang W Chang SF Chen HY Yu HS
Source: JDermatol 1992;19(1):1-11
Abstract:
A new human skin cell line, designated as CCFS-1/KMC, immortalized from human neonatal foreskin diploid fibroblast cells, has been subcultured successfully in vitro for more than 500 passages. This anchorage-dependent cell line possesses many common features of transformation such as morphological and cytoskeletal changes, hypotriploidy, infinite lifespan, increasing plating efficiency and saturation density, and decreasing serum requirement and population doubling time. Human papillomavirus (HPV) type 18 DNA was detected in the cell line before and after immortalization by the polymerase chain reaction (PCR) method. Tumorigenicity, however, was not demonstrated in vivo. The authors report that these immortalized human fibroblasts derived from neonatal HPV-18-DNA-contained diploid fibroblasts possess double minute chromosomes (DMs), a karyotypic aberration usually found in cancer cells."</I>
Interesting that one, some babies appear to have already got HPV??? It's contaminated the cell line, it would be interesting to know if it manages to get into the end products.
2. <I>"Safety evaluation of human living skin equivalents.
Authors: Nemecek GM Dayan AD
Source: Toxicol Pathol 1999;27(1):101-3.
..The resulting product has the appearance and handling characteristics of human skin. Safety evaluation of LSEs begins with insuring that foreskins are obtained only from healthy infants whose mothers are negative for a panel of adventitious agents."</I>
I wonder if the mothers realise what is going on here, they're being checked out to make sure their babies are suitable before harvest....
3. <I>"Use of fluorescent redox indicators to evaluate cell proliferation and viability. Authors: Rasmussen E
Source: In Vitro Mol Toxicol 1999;12(1):47-58.
...Cultures of human neonatal foreskin fibroblasts were exposed to equal concentrations of the two dye solutions in the cell culture media..."</I>
4. <I>"USE OF AN IN VITRO SKIN MODEL FOR DETERMINING EPIDERMAL AND DERMAL CONTRIBUTIONS TO IRRITANT RESPONSES
Authors: Slivka SR Zeigler F
Source: J Toxicol, Cutaneous Ocul Toxicol1993;12(1):49-57
.... An in vitro skin model was used to study the responses of epidermal and dermal cells to irritants. This model consists of neonatal foreskin fibroblasts grown into a three-dimensional dermal structure on a nylon mesh....."</I>
5. <I>"IN VIRTO TOXICITY OF VARIOUS CLASSES OF TEST AGENTS USING THE NEUTRAL RED ASSAY ON A HUMAN TRHEE-DIMENSIONAL PHYSIOLOGIC SKIN MODEL
Authors: Triglia D Braa SS Yonan C Naughton GK
Source: In Vitro Cell Develop Biol 1991;27A(3):239-244. (28 REFS)
Toxicity data were presented for the following classes of test agents: 15 detergents, five alcohols, ten metal chlorides, five perfumes and colognes, four shampoos, three conditioners, three moisturizers, three pesticides, and four antimicrobial preservatives. Dose dependent cytotoxic responses were observed for all test agents in each category. A new three dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin derived fibroblasts and epidermal keratinocytes grown on nylon mesh were used to assess the toxicity of these agents. A slight modification of the published neutral red viability assay was used for endpoint determination. The potential utility of the human dermal fibroblast epidermal keratinocyte, three dimensional substrate as an in-vitro toxicology assay system was described. "</I>
http://toxnet.nlm.nih.gov/altbib.html[/URL]
In the search field I put in "neonatal Foreskin" 5 results:
Unfortunately, the search results are only held in a temp file, so they don't work when the search expires. I've given the information on how to get there, and I hope it will be possible for other people to easily pull the abstracts up.
1. <I>"CHARACTERIZATION OF AN IMMORTALIZED HUMAN CELL LINE DERIVED FROM NEONATAL FORESKIN DIPLOID FIBROBLASTS
Authors: Chiang LC Chiang W Chang SF Chen HY Yu HS
Source: JDermatol 1992;19(1):1-11
Abstract:
A new human skin cell line, designated as CCFS-1/KMC, immortalized from human neonatal foreskin diploid fibroblast cells, has been subcultured successfully in vitro for more than 500 passages. This anchorage-dependent cell line possesses many common features of transformation such as morphological and cytoskeletal changes, hypotriploidy, infinite lifespan, increasing plating efficiency and saturation density, and decreasing serum requirement and population doubling time. Human papillomavirus (HPV) type 18 DNA was detected in the cell line before and after immortalization by the polymerase chain reaction (PCR) method. Tumorigenicity, however, was not demonstrated in vivo. The authors report that these immortalized human fibroblasts derived from neonatal HPV-18-DNA-contained diploid fibroblasts possess double minute chromosomes (DMs), a karyotypic aberration usually found in cancer cells."</I>
Interesting that one, some babies appear to have already got HPV??? It's contaminated the cell line, it would be interesting to know if it manages to get into the end products.
2. <I>"Safety evaluation of human living skin equivalents.
Authors: Nemecek GM Dayan AD
Source: Toxicol Pathol 1999;27(1):101-3.
..The resulting product has the appearance and handling characteristics of human skin. Safety evaluation of LSEs begins with insuring that foreskins are obtained only from healthy infants whose mothers are negative for a panel of adventitious agents."</I>
I wonder if the mothers realise what is going on here, they're being checked out to make sure their babies are suitable before harvest....
3. <I>"Use of fluorescent redox indicators to evaluate cell proliferation and viability. Authors: Rasmussen E
Source: In Vitro Mol Toxicol 1999;12(1):47-58.
...Cultures of human neonatal foreskin fibroblasts were exposed to equal concentrations of the two dye solutions in the cell culture media..."</I>
4. <I>"USE OF AN IN VITRO SKIN MODEL FOR DETERMINING EPIDERMAL AND DERMAL CONTRIBUTIONS TO IRRITANT RESPONSES
Authors: Slivka SR Zeigler F
Source: J Toxicol, Cutaneous Ocul Toxicol1993;12(1):49-57
.... An in vitro skin model was used to study the responses of epidermal and dermal cells to irritants. This model consists of neonatal foreskin fibroblasts grown into a three-dimensional dermal structure on a nylon mesh....."</I>
5. <I>"IN VIRTO TOXICITY OF VARIOUS CLASSES OF TEST AGENTS USING THE NEUTRAL RED ASSAY ON A HUMAN TRHEE-DIMENSIONAL PHYSIOLOGIC SKIN MODEL
Authors: Triglia D Braa SS Yonan C Naughton GK
Source: In Vitro Cell Develop Biol 1991;27A(3):239-244. (28 REFS)
Toxicity data were presented for the following classes of test agents: 15 detergents, five alcohols, ten metal chlorides, five perfumes and colognes, four shampoos, three conditioners, three moisturizers, three pesticides, and four antimicrobial preservatives. Dose dependent cytotoxic responses were observed for all test agents in each category. A new three dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin derived fibroblasts and epidermal keratinocytes grown on nylon mesh were used to assess the toxicity of these agents. A slight modification of the published neutral red viability assay was used for endpoint determination. The potential utility of the human dermal fibroblast epidermal keratinocyte, three dimensional substrate as an in-vitro toxicology assay system was described. "</I>
MatTek Corporation manufactures all kinds of human tissue substitutes, all the ones ending in -Derm, and EpiOcular being derived from neonatal foreskin. It's interesting to see the other human tissues they use too, breast tissue, oral tissue from people undergoing tooth extraction.
http://www.mattek.com/pages/products[/URL]
---------------------------------------------------------------------
http://www.msnbc.com/modules/cosmeti...tent.asp?p=all[/URL]
<I>"Human collagen</I>
<I>What</I>
<I>Injectable collagen derived from newborn foreskin left over after circumcision. To create the product, fibroblasts — the cells that produce collagen — are isolated from the foreskin, and replicated in a culture where they are primed to pump out collagen. McGhan Medical Corp. has petitioned the FDA for approval of two formulations of the foreskin-derived collagen: Cosmoderm, which is thinner, and Cosmoplast, a thicker version.</I>
<I>Use</I>
<I>To fill fine lines, deep wrinkles and scars, and plump lips.</I>
<I>Duration</I>
<I>Three to six months.</I>
<I>Cost</I>
<I>It hasn’t been priced yet but doctors expect it to cost about the same as bovine collagen: $300 and up.</I>
<I>Pros</I>
<I>* No allergy test or waiting period required.</I>
<I>* Can be used on patients with a bovine allergy.</I>
<I>* Mixed with anesthetic so less painful than hylan gel or products derived from cadaver tissue. </I>
<I>Cons</I>
<I>* Potential side effects include: Redness, swelling, bruising, infection and small risk of allergic reaction. "</I>
*McGhan Medical Corp is now known as Inamed Aesthetics, http://www.inamed.com/corporate/[/URL]
http://www.inamed.com/products/facia...formation.html[/URL]
More info:
http://or-live.mediwire.com/main/Def...ArticleID=3757[/URL]
<I>Choices to considerOn the list of soon-to-be-marketed products are a series of bioengineered human collagen products from McGhan - CosmoDerm-I NST, CosmoDerm-II NST, and CosmoPlast NST. This material is derived from cultured neonatal foreskin using a single line cell source, and each of these products is said to have the same performance characteristics as their respective bovine collagen counterparts - Zyderm-I, Zyderm-II, and Zyplast. However, with the bioengineered human material, the need for skin testing is obviated.</I>
<I>A number of recently available filler agents, including Cymetra (Life-Cell), Fascian (Medical Aesthetics International), and Dermalogen (Collagenesis) are developed from allogeneic material obtained from tissue banks - an industry not currently under the regulation of the FDA.</I>
<I>Cymetra is a micronized form of Alloderm, which is acellular freeze-dried human cadaver dermis. The material for production of these agents is obtained only from American Association of Tissue Banks (AATB) guideline compliant facilities. </I>
(http://mediwire.healingwell.com/main...ArticleID=3757[/URL] same article)
It's amazing the uses they put this material to, no wonder they don't want its production to stop!
-----------------------------------------------------------------------------------------------------------------------------
US Patents mentioning use of neonatal foreskins (search criteria - skin and alternative and animal and foreskin and neonatal - 497 results) :
Human foreskin cells suitable for culturing stem cells
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
Other searches bring up more (search criteria - organogenesis and foreskin - 62 hits):
Autogenic living scaffolds and living tissue matrices: methods and uses thereof
http://appft1.uspto.gov/netacgi/nph-...s+AND+foreskin[/URL])
Bioengineered tissue constructs and methods for producing and using thereof
http://appft1.uspto.gov/netacgi/nph-...s+AND+foreskin[/URL])
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I suppose we, in the rest of the world, should be grateful that American (and soon African) parents are so willing that their children's flesh can be harvested to provide so many useful materials, under "informed consent" too.
Circumcision makes no sense at all, until you look at American commercial interests..then it suddenly all clicks into place. Follow the money.
http://www.mattek.com/pages/products[/URL]
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http://www.msnbc.com/modules/cosmeti...tent.asp?p=all[/URL]
<I>"Human collagen</I>
<I>What</I>
<I>Injectable collagen derived from newborn foreskin left over after circumcision. To create the product, fibroblasts — the cells that produce collagen — are isolated from the foreskin, and replicated in a culture where they are primed to pump out collagen. McGhan Medical Corp. has petitioned the FDA for approval of two formulations of the foreskin-derived collagen: Cosmoderm, which is thinner, and Cosmoplast, a thicker version.</I>
<I>Use</I>
<I>To fill fine lines, deep wrinkles and scars, and plump lips.</I>
<I>Duration</I>
<I>Three to six months.</I>
<I>Cost</I>
<I>It hasn’t been priced yet but doctors expect it to cost about the same as bovine collagen: $300 and up.</I>
<I>Pros</I>
<I>* No allergy test or waiting period required.</I>
<I>* Can be used on patients with a bovine allergy.</I>
<I>* Mixed with anesthetic so less painful than hylan gel or products derived from cadaver tissue. </I>
<I>Cons</I>
<I>* Potential side effects include: Redness, swelling, bruising, infection and small risk of allergic reaction. "</I>
*McGhan Medical Corp is now known as Inamed Aesthetics, http://www.inamed.com/corporate/[/URL]
http://www.inamed.com/products/facia...formation.html[/URL]
More info:
http://or-live.mediwire.com/main/Def...ArticleID=3757[/URL]
<I>Choices to considerOn the list of soon-to-be-marketed products are a series of bioengineered human collagen products from McGhan - CosmoDerm-I NST, CosmoDerm-II NST, and CosmoPlast NST. This material is derived from cultured neonatal foreskin using a single line cell source, and each of these products is said to have the same performance characteristics as their respective bovine collagen counterparts - Zyderm-I, Zyderm-II, and Zyplast. However, with the bioengineered human material, the need for skin testing is obviated.</I>
<I>A number of recently available filler agents, including Cymetra (Life-Cell), Fascian (Medical Aesthetics International), and Dermalogen (Collagenesis) are developed from allogeneic material obtained from tissue banks - an industry not currently under the regulation of the FDA.</I>
<I>Cymetra is a micronized form of Alloderm, which is acellular freeze-dried human cadaver dermis. The material for production of these agents is obtained only from American Association of Tissue Banks (AATB) guideline compliant facilities. </I>
(http://mediwire.healingwell.com/main...ArticleID=3757[/URL] same article)
It's amazing the uses they put this material to, no wonder they don't want its production to stop!
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US Patents mentioning use of neonatal foreskins (search criteria - skin and alternative and animal and foreskin and neonatal - 497 results) :
Human foreskin cells suitable for culturing stem cells
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
http://appft1.uspto.gov/netacgi/nph-...)+AND+foreskin[/URL])
Other searches bring up more (search criteria - organogenesis and foreskin - 62 hits):
Autogenic living scaffolds and living tissue matrices: methods and uses thereof
http://appft1.uspto.gov/netacgi/nph-...s+AND+foreskin[/URL])
Bioengineered tissue constructs and methods for producing and using thereof
http://appft1.uspto.gov/netacgi/nph-...s+AND+foreskin[/URL])
------------------------------------
I suppose we, in the rest of the world, should be grateful that American (and soon African) parents are so willing that their children's flesh can be harvested to provide so many useful materials, under "informed consent" too.
Circumcision makes no sense at all, until you look at American commercial interests..then it suddenly all clicks into place. Follow the money.
sorry, couldn't help itmusicmom said:
. "a LOT of a good thing can be wonderful" is a story of my life...
Surprising research results at AIDS conference</SIZE>[/FONT][/COLOR][/B]
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http://www.sfgate.com/cgi-bin/article.cgi?f=/c/a/2008/02/04/MNCVURII9.DTL[/COLOR][/SIZE]</SIZE>[/URL][/B][/COLOR]
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They finally admitted publically, that male circ increases risk of HIV transmition to women. Reading this, keep in mind that the authors are <U>EXTREMELY pro-circ</U>, those very people that came up with the whole 'circ fights HIV' nonsense. So, admitting this, <U>even in such a weak form, is a HUGE DEAL</U>.</SIZE>[/FONT][/COLOR]
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http://www.sfgate.com/cgi-bin/article.cgi?f=/c/a/2008/02/04/MNCVURII9.DTL[/COLOR][/SIZE]</SIZE>[/URL][/B][/COLOR]
<SIZE size="150"></SIZE>
They finally admitted publically, that male circ increases risk of HIV transmition to women. Reading this, keep in mind that the authors are <U>EXTREMELY pro-circ</U>, those very people that came up with the whole 'circ fights HIV' nonsense. So, admitting this, <U>even in such a weak form, is a HUGE DEAL</U>.</SIZE>[/FONT][/COLOR]
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we could use some education here as well about many things, circ and AIDS are among themSuperMario said:
I chose not to circumcise my boy because the doctor said he didn't have excessive skin - besides, just because you are circumcised, it doesn't mean that you don't have to clean "it"
There is <U>no such thing</U> as excessive skin on males genitals as there is no such thing on female's one! Everything is there for a REASON!gagazine.com said:I chose not to circumcise my boy because the doctor said he didn't have excessive skin - besides, just because you are circumcised, it doesn't mean that you don't have to clean "it"
I'm NOT attacking anyone. Correcting does NOT mean attacking.[/B][/SIZE]</SIZE>[/FONT][/COLOR]Kaytee said:
I just corrected the doctor who was totally wrong calling foreskin (of any length) an excessive skin. This is just as ridicules as calling that a girl's clitoral hood. SAME VERY tissue with the same purpose (protection and sexual pleasure) in both male and female genitals; there is NOTHING excessive about it.[/B][/SIZE]</SIZE>[/FONT][/COLOR]